Chromatography

A thorough understanding of the chromatographic process, is the foundation of robust and reliable methods

Topics

  • Understanding the general chromatographic process
  • The Reversed Phase mechanism
  • The HILIC mechanism
  • The Size Exclusion mechanism
  • Affecting retention and selectivity
  • Peak distortion
  • Intermezzo: pH / analyte charge / retention
  • Scaling Chromatography
  • Trouble shooting
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