Chromatography
A thorough understanding of the chromatographic process, is the foundation of robust and reliable methods
![](https://hendrikslabimprovement.com/wp-content/uploads/2023/02/Gradient-Chromatogram.png)
Topics
- Understanding the general chromatographic process
- The Reversed Phase mechanism
- The HILIC mechanism
- The Size Exclusion mechanism
- Affecting retention and selectivity
- Peak distortion
- Intermezzo: pH / analyte charge / retention
- Scaling Chromatography
- Trouble shooting